alexa Abstract | Stability indicating RP-HPLC method for simultaneous estimation of Amlodipne and Perindopril in bulk and formulation

Journal of Chemical and Pharmaceutical Research
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Abstract

An isocratic, reversed phase liquid chromatographic method was developed for the simultaneous quantitative determination of amlodipine and perindopril in bulk and combined dosage form. The chromatographic separation was achieved on Kromasil octadecylsilane (150 x 4.6 mm, 5 μ) column, with mobile phase comprising disodium hydrogen phosphate buffer pH 3 and acetonitrile in the ratio of 32:68. The flow rate was maintained as 1.0 ml/min with 30 °C column temperature and effluents were monitored at 218 nm. The separation was achieved with less than 5 min. The retention times of amlodipine and perindopril were 2.899 min and 2.288 min, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity and robustness. Linearity was observed in the concentration range of 12.5-75 μg/ml for amlodipine and 10-60 μg/ml for perindopril. Correlation co-efficient for amlodipine and perindopril was found to be 0.999 and 0.999, respectively. Recovery of amlodipine and perindopril in formulations was found to be in the range of 99.84-100.14 % and 99.38-99.97 %, respectively, confirms the non-interferences of the excipients in the formulation. The % RSD below 2.0 shows the high precision of the proposed method. Due to its simplicity, rapidness and high precision, the method was successfully applied to the estimation of amlodipine and perindopril in combined dosage form.



Author(s): G Saravanan D Asha Jyothi Kommara I Sri Krishnanjaneyulu D Visagaperumal

Keywords

Amlodipine, Perindopril, RP-HPLC method, Method development, Method validation., Amlodipne and Perindopril

 
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