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The aim of this paper was to study the active substances in Taxus ???? uspidate and their antiosteosarcoma effect. Preparative chromatography, column chromatography and preparative HPLC were used for the structural characterization of isolated active substances. MTT assay, electron microscopy and flow cytometry were applied to detect the anti-osteosarcoma effect of paclitaxel. Five compounds were isolated. Different concentrations of paclitaxel test solutions all had an inhibitory effect on MG-63 cells. With the increase in action time, the survival rate of tumor cells declined. Under an inverted microscope, MG-63 cells; which were treated with paclitaxel for 24 h, partially turned round and were floating. Cell volumes diminished, after which the cells immediately detached from the adjacent cells, and apoptosis occurred. Osteosarcoma is one of the most common bone tumors in the adolescent group, and Taxus uspidate has a significant anti-osteosarcoma activity.
Taxus cuspidate, flow cytometry, MG-63 cell