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Short Communication Open Access
The seeds of C-429, R-552, RLC-4, RLC-6 and T-397 varieties of Linseed, were extracted with chloroform-methanol (2:1,v/v) to yield the total lipids (TL) in 43.9, 46.3, 42.1, 43.5 and 45.2 percent respectively. The TL were fractionated by silicic acid column chromatography into neutral lipids (NL) (87.8-89.6%), glycolipids (GL) (5.8-6.6%) and phospholipids (PL) (3.8-5.8%). The NL, upon being subjected to preparative TLC, were separated into monoacylglycerol, diacylglycerol, triacylglycerol, free fatty acids, sterols, steryl esters and hydrocarbons. The fatty acid composition of all the lipid materials, as determined by GLC, revealed the major fatty acids to be linolenic, linoleic, oleic, stearic and palmitic acids. The fatty acids remained static qualitatively but variability between the lipids and varieties was observed.
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Author(s): R R Khotpal A S Kulkarni H A Bhakare