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Research Paper Open Access
This study focused on profiling the major components of a complex traditional Chinese herbal preparation composed of seventeen herbs, Fangfeng Tongsheng Pills by HPLC with photodiodearray detection. Based on the optimized protocol, the HPLC fingerprint profiles were established. Within the chromatographic fingerprint, sixteen identified bioactive compounds belonging to iridoid, anthraquinones, flavonoids, triterpenoid, phenol, phenolic acids, alkaloid and chromones were successively separated and twelve was quantitatively determined. The linearity and range, LOD, LOQ, precision, accuracy and stability of the assay were validated according to Chinese pharmacopoeia. The validated data showed that the method was suitable for quality evaluation of the valuable medicine listed in Chinese pharmacopoeia by qualitative and quantitative information in one chromatographic fingerprint.
Fangfeng Tongsheng Pills, HPLC, multicomponent quantification, Chinese herbal preparation