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The optimal conditions for preparation of total phenolic acids in Phyllanthus emblica, and to study their inhibitory effect on human hepatoma cells have been investigated. The conditions for extraction of phenolic acid constituents in Phyllanthus emblica were investigated, and single factor and orthogonal tests were performed on conditions such as extraction solvent, extraction temperature, extraction time and solid-liquid ratio, respectively. To determine the inhibition ratio of drugs on cancer cells in different concentrations and periods by MTT method, and make quantitative determination for apoptosis by Flow cytometry to explore anticancer mechanism of drugs. The optimal extraction process of total phenolic acids in Phyllanthus emblica was extraction at 50? for 2 h with 50% ethanol. The total phenolic acids with different concentrations have remarkable inhibiting effects on human Hepatocarcinoma cell SMCC- 7721. The growth inhibitory rate of cells in test group is on the rise and depends on density with the increasing of medicine concentrations. Flow cytometry testing results show that the total phenolic acids in Fructus Phyllanthi can stop human Hepatocarcinoma cell SMCC-7721 in S phase and the effect is obvious. Conclusion the total phenolic acids in Fructus Phyllanthi have significant inhibiting effects for the proliferation of Hepatocarcinoma cell. It can induce the cell apoptosis, and has better anticancer effects.
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Author(s): Kun Li Taowen Pan Shanshan Huang Chuanxun Li Fei Wang
Phenolic acids, Hepatocarcinoma Cell, MTT, Flow Cytometry.