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Original Articles Open Access
The outbreak of infectious disease caused by pathogenic bacteria and the emergence of antibiotic resistant bacteria has created a demand for new antimicrobial agents. Nowadays metal nanoparticles are of great interest due to unique optical and chemical properties. Amongst these, silver nanoparticles (AgNPs) have strong bactericidal effects. Green synthesis of AgNPs is cost effective and does not produce harmful toxins. Tamarind seed coat is rich in polyphenols and tannins which are the major compounds required for the synthesis of AgNPs. The objective of the project is, to synthesize AgNPs using the aqueous seed coat extract of tamarind and observe its antibacterial activity against Escherichia coli and Bacillus cereus. Phytochemical tests were done to confirm the presence of polyphenols, flavanoids, tannin and saponin in aqueous extract and the results were positive. TPC assay was done to estimate the amount of phenol present in aqueous extract in terms of gallic acid equivalence (GAE) and concentrations obtained were 80g and 108g GAE/100g of dry weight for 0.2 and 0.4 mg/ml concentrations. The formation of AgNP was confirmed by obtaining an intense peak in UV-visible spectrophotometer at 423 nm. XRD was done to confirm the average size of the AgNPs synthesized and it was found to be 12.73 nm. Antibacterial activities were determined by measuring the zone of inhibition obtained for B.cereus (26mm) and E.coli (15mm). The study concludes that, AgNPs synthesized using aqueous seed coat extract effectively inhibits bacteria and can be used to prepare drugs that can target against these bacteria.
Tamarind Seed Coat, Total Phenol Content, Silver Nanoparticles, Antibacterial, XRD, silver nanoparticles