alexa Abstract | The Effects of Fenton Type (Fe+2/H2O2) Oxidation System on Lipid and Protein Oxidation of Grey Mullet (Mugil cephalus)

Journal of
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)


Oxidative damages of lipid and protein in grey mullet (Mugil cephalus) were investigated in vitro by using Fenton reactants (Fe+2SO4/H2O2) to generate hydroxyl radical formation. Thiobarbutiric acid–reactive substances (TBARs, mg maonladehyde/ kg) were used to determine free radicalmediated oxidation of lipids. The oxidative modification of proteins was confirmed by carbonyl formation using with 2,4-dinitrophenylhydrazine (DNPH) and DNPH labeled of oxidized proteins by separated Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Lipids Oxidation of grey mullet with Fe+2/H2O2 gave a noticeable increase in TBARs value during 5h incubation. Protein carbonyl content decreased during the first 3h of incubation time on treatment with free radical generating system and then increased until 5th hours. At 3rd hours of incubation the intensity of the bands near 100 kDa and 150 kDa decreased and the bands above 50 kDa disappeared at 5th hours of incubation after exposure to Fe+2/H2O2. These data suggest that free radical generating system can alter the structure of a protein by leading to protein denaturation and increase in lipid oxidation by leading to development of off- flavors and off-odors during storage, which cause quality loss and limited storage life.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Tokur Bahar


Iron catalyzed oxidation, Carbonyl content, SDS-PAGE, Malonaldehyde, Grey mullet, Fish genetics and genome, Fisheries management, Aquatic health management

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version