alexa Abstract | The Green Fluorescent Protein (GFP) is a Vital Visual Marker in Citrus Transgene Research

Electronic Journal of Biology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Abstract

pGREEN is a flexible Ti binary vector for Agrobacterium mediated transformation system [1]. The advantage of this vector is that optional combinations of selection and reporter gene cassettes can be optimally assembled to meet different transformation requirements. gfp (green fluorescent protein) gene driven by 35S-CaMV promoter was used as a reporter gene to enable transformant detection in vivo and the REDExtract-N-Amp Plant PCR Kit was applied as a high throughput approach for screening large amount of regenerated transformed plants in this report. With the designated vector pGK assembled using pGreen0029 and 35S-GFP, different phenotypes of transformation chimerism were observed and average transformation rates 7.75% and 2.03 % were acquired in term of consistent PCR results between the selectable and the reporter gene for empty vector and inserted recombinants respectively in transformation events of citrus grapefruits.

To read the full article Peer-reviewed Article PDF image

Author(s): Qifa Zheng Chunxian Chen Shu Huang Xu Xiang Young A Choi Fred G Gmitter Jr

Keywords

Agrobacterium, transformation, citrus, GFP (green fluorescent protein), PCR

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords