alexa Abstract | The Indian Medicinal Plant Giloe (Tinospora cordifolia) induces Cytotoxic Effects by Damaging Cellular DNA in HeLa Cells: A Comet Assay Study

Trends in Green Chemistry
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access


Environmental science is moving through a dreaded and drastic crisis. Tinospora cordifolia or giloe is used in Ayurvedic system of medicine for the treatment of many diseases. Our earlier studies have shown that giloe exerts cytotoxic effect in vitro and in vivo, which stimulated us to understand the mechanism of cell death is HeLa cells receving various concentrations of giloe. Therefore, induction of molecular DNA damage by different concentrations of dichloromethane extract of giloe, Tinospora cordifolia (TCE) was studied in HeLa cells by alkaline comet assay and the DNA damage induced by TCE has been expressed as olive tail moment (OTM). Incubation of HeLa cells with TCE for 4 h caused greater DNA damage as indicated by increased OTM when compared with 2 h treatment and therefore 4 h exposure time was considered as an optimum duration for TCE treatment. Exposure of HeLa cells to 0, 1, 2, 4, 6 or 8 μg/ml TCE resulted in a concentration dependent elevation in the DNA damage and the lowest concentration of 1 μg/ ml TCE increased the baseline DNA damage approximately by 10 folds, whereas 8 μg/ml TCE, the highest concentration tested caused an approximate 68 folds elevation in the DNA damage, when compared with the untreated control.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Ganesh Chandra Jagetia Shaival K Rao


Environmental Chemistry, Microwave Chemistry, Biocatalysis

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version