alexa Abstract | The minicell generation in Escherichia coli harboring minD of Lactobacillus

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access


Minicells have been studied as drug delivery with highly significant anti-tumor effects in vivo. Minicells were produced by mutant bacteria without min locus. In this study, the minicell generation was done in the glucose condition in combination with different antibiotic concentrations when culturing the Escherichia coli BL21(DE3)plysS harboring pET 21(a+) and pET 28(a+) inserted with minD gene of Lactobacillus acidophillus VTCC-B-871. The minD gene gives a filamentous phenotype in 1% glucose and ampicillin at concentration at 100mg/ml for the host cells harboring pET 21(a+)/minD and kanamycin at 50mg/ml for the host cells harboring pET 28(a+)/minD. However, the filaments changed into minicells when chloramphenicol presented in the culture with the long incubation time to 24 hours. Especially, glucose could induce the expression of MinD. The morphology differentiation was observed by microscope. The study supported the minicell generation with high rate and enough amount for drug delivery.

To read the full article Peer-reviewed Article PDF image

Author(s): Ly Ha Vinh Doan Huu Nguyen and Tu Nguyen


glucose, Lactobacillus acidophilus VTCC-B-871, minD gene, minicell, Escherichia coli

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us