alexa Abstract | Three-dimensional cultures of dissociated myenteric plexus as a model for the development of the enteric nervous system

Biomedical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Abstract

Failures in the development of the enteric nervous system (ENS) can lead to severe dis-eases, such as Hirschsprung`s disease or any other dysganglionosis. Trying to imitate the de-velopment of the ENS, the myenteric plexus, in vitro might give us a better understanding of the underlying problems and the chance to develop new diagnostic and even therapeutic ap-proaches. Myenteric plexus of newborn rats was isolated by enzymatical digestion, dissociated and cultivated either on glass coverslips, or in a three-dimensional gel of extracellular-matrix-components. Supplementing the culture media with the neurotrophins Glial-cell-line-derived-neurotrophic-factor (GDNF) or brain-derived-neurotrophic-factor (BDNF), alone or in com-bination, lead to differences in the growth behaviour of the individual culture. GDNF increased the survival, as well as the neurite outgrowth of the neurons, while BDNF had no obvious effect when administered alone. Only in combination with GDNF it in-creased the branching patterns of the neurites, indicating a higher degree of differentiation. These phenomena were seen on glass, as well as in the gel cultures. The only difference was, that cultures grown in the gels formed secondary ganglia which were similar to what is seen in more severe cases of dysganglionosis, while there was no specific reaggregation on glass. The degree of “artificial hypoganglionosis” varied, depending on the factors which were supple-mented to the media. Cultivating dissociated myenteric plexus in a three-dimensional environment seem to be a valuable model to imitate the development of the ENS, and so to investigate the effect respec-tively the lack of certain factors for the development of an intact and well working gastrointes-tinal innervation.

To read the full article Peer-reviewed Article PDF image

Author(s): Stefan HollandCunz Cornelia Hagl HeinzJrgen Krammer Matthias Lintz Luuk Wessel and KarlHerbert Schfer

Keywords

enteric nervous system, dysganglionosis, neurotrophins, three-dimensional tissue culture

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords