alexa Abstract | Time-kill kinetics and antibacterial activity of crude methanolic extract of Thymus daenensis Celak.

Biomedical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)


Thymus daenensis has been known to possess various medicinal properties, including antibacterial activity. This study was designed to evaluate and quantify killing kinetics and endpoint parameters of antibacterial activity of T. daenensis. A methanolic extract of T. daenensis was prepared and tested against several gram-positive and gram-negative pathogenic bacteria, using a colorimetric microdilution method. The findings revealed that except for Psudomonas aeroginosa, the 50% Minimum Inhibitory Concentrations (MIC50) inhibiting the growth of the gram-negative bacteria were significantly lower as compared to those of the gram-positive bacteria (p<0.05). By contrast, the endpoint MIC values were less different across the bacteria. Overall, the T. daenensis extract was most efficient against Bacillus cereus and Escherichia coli, whilst P. aeroginosa was the least susceptible one. The profiles of killing and re-growth of the bacteria were then assessed as functions of both time and the extract concentration (corresponding to MIC, ½ MIC, and ¼ MIC). E. coli and B. cereus were completely blocked at the concentrations corresponding to MIC at less than two hours after inoculation. This inhibition pattern remained unchanged until the end of the time course. At the concentrations corresponding to ½ × MIC, a rapid complete inhibition was observed for E. coli, B. cereus, Staphylococcus aureus, and Enterococcus faecalis. Overall, this quantified information on dose-response rate, endpoint parameters, and kinetic profiles may provide an initial step towards understanding in vitro pharmacodynamics of antibacterial activity of T. daenensis.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Sharareh Rezaeian Samaneh Attaran Hamid R Pourianfar


Thymus daenensis, Methanolic extract, Time-kill kinetics, Pharmacodynamics, #

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us