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Research Paper Open Access
The static culture of Tribulus terrestris Linn was established in Murashige and Skoog's1 medium supplemented with Indole acetic acid (IAA) 1 ppm, 2,4-dichlorophenoxy acetic acid (2, 4 D)1 ppm. and 25 ml of coconut water. The 8 months old callus was analysed for the presence of hecogenin and neotigogenin and were quantitatively estimated by spectrophotometric method.