alexa Abstract | Total phenolics content, flavonoids profiling and antioxidant activity of Lippia multiflora leaves extracts from Burkina Faso

Asian Journal of Plant Science & Research
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Research Article Open Access

Abstract

Polyphenolics, and particularly flavonoids are known and sought for their multiple biological properties. The present study aimed to assess the total phenolics (TP), total flavonoids (TF) contents and the antioxidant activity (AOA) of Lippia multiflora leaves extracts, an aromatic plant of subtropical Africa whose traditional practices reveal enormous potential. The dried leaves of Lippia multiflorawere extracted successively with hexane, dichloromethane, ethyl acetate and ethanol by cold maceration for 24 hours. After a phytochemical screening, the TPCwas determined using the Folin-Ciocalteu reagent, the TFC by aluminum chloride test and the AOA by the DPPH test and the lipid peroxidation inhibitionassay. The results showed that ethanolic crude extract had significant phenolics and total flavonoids contents and also good AOA. Furtherfractionation of this extract by column chromatography, using ethyl acetate - methanol (80: 20), afforded a fraction which AOA (IC50 = 23.68 μg/mL) is more important than those of crude extract (IC50 = 38.20 μg/mL) and vitamin C (IC50 = 80.33 μg/mL). This fraction also inhibited 29.19, 33.57 and 67.15 % lipid peroxidation at 250, 500 and 1000 μg/mL respectively. Lippia multiflora ethanolic extract may provide a source of natural antioxidants and these preliminary results allow further investigation of other biological activities including anti-cancer and antidiabetic properties to enhance the extracts of this plant in phytomedicine

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Author(s): Constantin M Dabire Remy K Bationo Adama Hema Roger C H Nebie Eloi Pale S P Dhanabal and Mouhoussine Nacro

Keywords

Lippia multiflora, polyphenolics, flavonoids, antioxidant activity, phytomedicine, Lippia multiflora, polyphenolics, flavonoids, antioxidant activity, phytomedicine

 
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