alexa Abstract | Type II Cyclic Guanosine Monophosphate-Dependent Protein Kinase Inhibits Vegf-A/Vegfr-2 Pathway Activation In Gastric Cancer Cells

Electronic Journal of Biology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Abstract

Our previous study found that Type II cyclic guanosine monophosphate-dependent protein kinase (PKG II) inhibited VEGF-induced tyrosine phosphorylation/ activation of VEGFR-2. But how does PKG II inhibit VEGFR-2 is not clear yet. The aim of this paper was to investigate the molecular mechanism of PKG II inhibition on VEGFR-2. Human gastric cancer cell line HGC-27 and Human Umbilical Vein Endothelial Cells (HUVECs) were infected with adenoviral construct encoding cDNA of PKG II (Ad-PKG II) to increase the expression of PKG II and treated with 8-pCPT-cGMP to activate the kinase. Trans-well migration assay results showed that PKG II inhibited VEGF-induced migration of gastric cancer cells. Tube formation assay results showed that PKG II inhibited VEGF-induced tube formation of HUVEC cells. Co- Immunoprecipitation results indicated that PKG II combined with VEGFR-2. Immunoprecipitation and Western blotting results showed that PKG II caused Serine/Threonine phosphorylation of VEGFR-2. Mutagenesis and Western blotting results showed that when Threonine 439 and Serine1231 of VEGFR-2 were mutated to Alanine which could not be phosphorylated, the inhibition of PKG II on VEGFR-2 disappeared. The results suggest that PKG II inhibits VEGF-induced activation of VEGFR-2, migration and tube formation through phosphorylating Threonine439 and Serine1231 of VEGFR-2.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Ying Wang Weihui Zhang Yan Wu Min Wu Hai Qian Yongchang Chen

Keywords

Type II cGMP-dependent protein kinase(PKG II), VEGF, VEGFR-2, Gastric cancer cells, Phosphorylation, Inhibition

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords