alexa Abstract | Validated HPTLC method for determination of dicloxacillin in simulated urine

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access


A simple, selective, and sensitive HPTLC method for the determination of Dicloxacillin in Simulated Urine was developed & Validated. The method utilizes Protein precipitation as the sample preparation technique. The HPTLC separation was achieved on the aluminum backed layer of silica gel 60 F254 using Toluene : methanol : Triethylamine (4:6:0.5% v/v/v) as mobile phase. The Detection wavelength used was 227nm. The chamber saturation time is 15min.Cefixime was used as an internal standard. The calibration curve was linear ( r2>0.99) through the range of 200-1000ng/band. The mean recovery was found to be 93.31% for Dicloxacillin. Quantification was achieved with HPTLC, over the concentration range of 200 to 1000 ng/band. Retention factor (Rf) for Dicloxacillin and Cefixime (IS) were 0.80 and 0.50 respectively. Dicloxacillin was found to be stable in simulated urine. The method showed acceptable values for accuracy, precision, recovery and stability.

To read the full article Peer-reviewed Article PDF image

Author(s): Prashant U Tompe Madhura V Dhoka Mrinalini C Damle and Ashwini R Madgulkar


HPTLC, simulated Urine matrix, Dicloxacillin, Cefixime, Protein Precipitation, HPTLC

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us