alexa Abstract | Validated HPTLC Method for Simultaneous Estimation of Ramipril and Metolazone in Bulk Drug and Formulation

Der Pharmacia Sinica
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access


This paper describes a new, simple, precise, and accurate HPTLC method for simultaneous estimation of Ramipril and Metolazone as the bulk drug and in tablet dosage forms. Chromatographic separation of the drugs was performed on aluminum plates precoated with silica gel 60 F254 as the stationary phase and the solvent system consisted of toluene : ethyl acetate : methanol : glacial acetic acid (4 : 4 : 1 : 0.2 v/v/v/v). Densitometric evaluation of the separated zones was performed at 223 nm. The two drugs were satisfactorily resolved with RF values 0.33 ± 0.02 and 0.59 ±0.02 for Ramipril and Metolazone respectively. The accuracy and reliability of the method was assessed by evaluation of linearity (600-2100 ng/spot for Ramipril and 100-350 ng/spot for Metolazone, precision (intra-day % RSD was 1.28 – 1.58 and inter-day % RSD was 1.14 – 1.83 for Ramipril and intra-day % RSD was 0.67 – 1.03 and inter-day % RSD was 0.49 – 1.18 for Metolazone), accuracy 99.44 ± 0.15 for Ramipril and 99.85 ± 0.39 for Metolazone), and specificity in accordance with ICH guidelines.

To read the full article Peer-reviewed Article PDF image

Author(s): Jitendra A Wayadande Ramkumar Dubey Vidhya K Bhusari and Sunil R Dhaneshwar


Thin layer chromatography, Densitometry, Validation and Quantification, Ramipril and Metolazone.

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us