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This work is concerned with application of simple, economical, precise, accurate and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of Valsartan potassium (VP) and Amlodipine besylate (AB) on RP C-18 Column (Inertsil ODS-2, 150 x 4.6 mm) using Methanol: Water (62:38), pH adjusted to 3.0 with O-phosphoric acid as mobile phase at a flow rate of 1.4 ml/min and the detection wavelength was 230 nm. The retention time for VP and AB was found to be 6.0 and 3.5 min, respectively. Proposed method was validated for precision, accuracy, linearity range, robustness and ruggedness.
Valsartan potassium, Amlodipine besylate, Reverse Phase High Performance Liquid Chromatography, Inertsil, retention time, C-18 column.