alexa Abstract | Value of mycobacterium tuberculosis direct assay in early diagnosis of extrapulmonary tuberculosis.

Biomedical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Abstract

The objective of the present study is to evaluate in situ detection of living mycobacterium tuberculosis rRNA using Mycobacterium Tuberculosis Direct Assay (MTD) and its clinical significance in early diagnosis of extrapulmonary tuberculosis. 86 patients having been diagnosed to have extrapulmonary tuberculosis including tuberculous peritonitis (n=22), lymphatic tuberculosis (n=21), tuberculous meningitis (n=15), HIV-associated tuberculosis (n=13), nephrotuberculosis (n=9), spinal tuberculosis (n=2), cutaneous tuberculosis (n=13), parotid tuberculosis (n=1), chest wall tuberculosis (n=1), intestinal tuberculosis (n=1) were recruited from Shanghai Public Health Clinical Center from June to November in 2010, 105 extrapulmonary specimens including CSF, puncture fluid, drainage, pleural fluid, urine, secrete, ascites, lymphatic tissue and marrow were collected from those patients. The samples were examined using acid fast stain, solid culture, liquid culture and MTD in parallel. In MTD, the target segments of MTB rRNA in either cultures or clinical specimens were amplified prior to be qualitatively detected with hybridization protection assay (HPA). The sensitivity of MTD, acid fast stain, liquid culture and solid culture was 48.6%, 41.9%, 20.0% and 14.3% respectively. MTD’s sensitivity was higher than the others and its specificity was 100%. MTD rRNA detection is an effective, rapid, convenient, sensitive and reliable method in early diagnosis of extrapulmonary TB.

To read the full article Peer-reviewed Article PDF image

Author(s): Huizhang Zhang Qiang Fang Jian Guo Yong Shen Suihua Lu Xiangnan Hu Guilin Deng Wenjuan Wu

Keywords

Mycobacterium tuberculosis, rRNA, MTD, Extrapulmonary TB

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us