alexa Abstract | Whole-mount immunostaining protocol for screening of spatial organizations of cerebellar compartmentation

Current Neurobiology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)


This paper introduces a protocol for whole-mount immunostaining adapted for the adult mouse cerebellum. This technique was originally described by Sillitoe and Hawkes (J His-tochem Cytochem 50:235-244, 2002) for screening the parasagittal arrangements of cere-bellar compartmentation antigens, i.e., zebrin II and heat shock protein (HSP25). We fur-ther optimized this technique to apply to heat-induced antigen retrieval, which provides a more rapid and efficient way to perform whole-mount immunostaining of adult tissues (Sawada and Sun, Curr Neurobiol 1:21-24, 2010). This approach allows for the visualiza-tion of spatial organizations of cerebellar examinations of the normal topography, particu-lar components of olivocerebellar tracts, and patterning defects caused by mutations without 3D reconstruction of immunostained serial sections.

To read the full article Peer-reviewed Article PDF image

Author(s): Kazuhiko Sawada and Emi Ochiai


Immunohistochemistry, Zebrin II, HSP25, CRF, Ca2+ Channelopathy, Rolling mouse

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version