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Invertebrates host numerous bacteria with interactions ranging from pathogenesis to symbiosis. Wolbachia is a ubiquitous, maternally inherited α-proteobacterium that causes a wide range of reproductive abnormalities in its hosts. In this paper, using polymerase chain reaction assay based on specific amplification of the 16s rRNA Wolbachia gene fragments, we recorded 100% infection in the workers of the honeybee subspecies Apis mellifera carnica collected at the Honeybee Research unit, Bremen, Germany, confirming that they are abundant in the workers of the honeybees screened
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Author(s): Mahesh Pattabhiramaiah Dorothea Brueckner Karl Paul Witzel and MSReddy
Apis mellifera carnica, PCR, Wolbachia, 16S rRNA gene.