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Research Article Open Access
Bacteriorhodopsin (bR) is a transmembrane protein which can exist in a variety form of chemical states. By choosing two states, O state for binary zero and Q state as binary one, it is possible to use this protein as a memory device. In order to increase O state life time and follow that Q state the new matrix was design to make a bR film. Thus, different ratios of Gelatin-polyvinyl alcohol matrices weight/volume were provided in order to find the optimal condition to light absorption. In addition, triethanolamine (TEA) was also used as photosensitizing chemical additive to film forming suspension. It found that the bR film matrix of 0.0005% (w/v) is the best ratio for bR light absorption. Furthermore, TEA changed both absorption and the lifetime of M intermediate. After wards, bR film was chemically immobilized on glassy surface and then illuminated by laser beam in different times. Radiation of bR films in various intervals after chemical immobilization demonstrated that bR ground state; M, P, and Q intermediates of bR photocycle are existed.
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Author(s): Soraya Mohammadi Ahmad MolaeiRad and Morteza Khabiri
Protein memory, Bacteriorhodopsin (bR), Photocycle, Laser beam