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Original Articles Open Access
Xylanases hydrolysis β-1,4-xylan of hemi-cellulose that exist in diverse forms among bacterial strains. Each strain specific xylanase display variation for xylan hydrolysis under specific environment like as extremophillic in particular to thermophiles, alkaliphiles, and acidiphiles. In this study, activity of xylanases secreted by six Bacillus strains i.e. B. amyloliquefaciens, B. cereus, B. pumilus, B. licheniformis, B. subtilis 168, B. thuringiensis in BXN liquid (supplemented with xylan as major nutrient or carbon source) medium were assayed under thermo-alkaliacidiphilic conditions to search its superior phenotype. In SDS-PAGE, higher total exoproteins in nutritive liquid medium of B. subtilis 168, B. amyloliquefaciens and B. pumilus and cell growth rate remain higher inB. subtilis 168 to B. pumilus and B. cereus. Best xylanolytic activity was shown by B. pumilus, B. licheniformis and B. cereus at neutrophilic conditions while does not remain persistent under alkaline, acidophilic and thermophilic (50°C) conditions except B. pumilus. For industrial point of view, xylanase production from these strains might be useful but required in relatively better quality and quantity. This lack of cellulose free xylanase availability could be permissibly fulfilled by non-pathogenic B. subtilis 168 through recombinant biotechnology. Production of xylanase of B. licheniformis or B. pumilus with B. subtilis 168 as a model system could be usefulfor industrial revolutionization.
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Author(s): Ikramul Haq
Bacillus strains, xylanase, gene sequences, reducing sugars, proteomics, evolutionary tree.