alexa Abstract | Achievement, Challenge and Future Perspective of BAC Library Technology with a Focus on its Application to Soybean Genomics Researches
ISSN: 2168-9849

Cloning & Transgenesis
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Abstract

Large genomic DNA insert-containing libraries e.g. bacterial artificial chromosome (BAC) libraries have made great contributions to advanced genomic studies, such as genome-wide sequencing, syntenic analysis between genomes, physical mapping, and positional cloning of important QTL genes in soybean or other legume. As recent advances in next-generation sequencing (NGS) technology, application of BAC or other large insert-containing library has become less demanding. We briefly review the history and applications of soybean BAC library technology. The challenge and future prospective of soybean BAC library technology in the post genomic-era are also discussed.

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Author(s): Hongyan Wu, Zhengjun Xia and Kyuya Harada

Keywords

Bacterial artificial chromosome, Genome, Library, Gene cloning, Soybean, Gene targeting,Gene expression, gene therapy

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