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Research Article Open Access
In order to study the antimicrobial activity of Cold Water, Hot Water, Methanol, Acetone, Chloroform, Diethyl ether, Xylene and Petroleum ether extract of three Indian spices such as Piper nigrum L., was tested against infectious disease causing bacterial pathogens Staphylococcus aureus and Staphylococcus epidermidis using the Agar Well diffusion method. It was observed that hot water, cold water, Methanol, Acetone, Chloroform, xylene, Diethyl ether and Petroleum ether showed activity against both Bacteria. The extract of Pimenta dioicas in different solvents showed maximum activity against Staphylococcus aureus. The results confirmed presence of antibacterial activity in the selected Indian spices against the human pathogenic organisms.
Antibiotic Profiling, Well diffusion method, Sub-culture, Inhibition Zone., Applied Sciences,Biochemistry,Biogenetic Engineering,Biomedical Engineering,Botany,Chromatography Techniques.