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Research Article Open Access
The hydrodistilled essential oil of leaves of Sequoia sempervirens (Lamb.) belonging to family Cupressaceae was analyzed by GC/MS. Thirty six compounds were identified representing 95.62% of the total oil containing α-phellandrene (29.60%), dl-limonene (15.60%), α-pinene (8.65%) and terpinene-4-ol (3.5%) as major components. The studied essential oil showed a dose-dependent antioxidant activity using 2, 2-diphenyl-1-picrylhydrazyl assay (IC50: 32.2 μg/mL). The antimicrobial activity of S. sempervirens leaves oil was screened against Gram-positive and Gram-negative bacteria and fungi. The strongest antibacterial effects of oil were on Bacillus subtilis, Trichophyton mentagrophytes and Staphylococcus aureus. S. sempervirens oil showed potent cytotoxic activity against the tested carcinoma cell lines; hepatocellular (HEPG2), colon adenocarcinoma (HCT-116) and breast (MCF7) comparing to doxorubicin.
S. sempervirens, GC/MS, Antioxidant, Antimicrobial, Cytotoxic, Aromatic Compounds, Aromatic Crops, Aromatic Flowers