alexa Abstract | Frequency of Rapid Growing Mycobacteria among Tuberculosis Suspected Patients in Basra- Iraq
ISSN: 0974-8369

Biology and Medicine
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access


Objective: The purpose of this study was to estimate the frequency of rapid growing mycobacteria among tuberculosis suspected patients in Basra governorate and study their resistance to drugs.

Methods: A total of 150 sputum samples were obtained from 150 suspected patients who attended the Advisory Clinic for Chest Diseases and Respiratory (ACCDR) in the Basra Governorate from 01/03/2013 to 1/02/2014. Smears were stained with the Ziehl Neelsen technique and specimens were inoculated on Lowenstein Jensen medium, Identification to species level was achieved on the basis of the growth characteristics, pigment production and conventional biochemical tests. Drug susceptibility was tested to rifampicin, ethambutol, pyrazinamide, isoniazid, and streptomycin using the proportional method.

Results: From 150 sputum samples, 23 isolates were Mycobacterium tuberculosis (MTB) (15.33%) and 16 (10.66%) were nontuberculous mycobacteria, seven isolates of them (43.75%), 2 males and 5 females, mean age 40 years, were identified using biochemical tests as rapid growing mycobacteria of which 4 (25%) as M. chelonae, 2 (12.5%) M. abscessus and 1 (6.2%) M. smegmatis. In addition to that, the bacteria were successfully differentiated by Duplex-PCR, as MTB and NTM based on amplification of rpoB gene sequences. Sequencing of 16S rDNA showed matching with 6 biochemically identified ones, and one of the 4 M. chelonae was M. chitae. Drug susceptibility testing showed that one M. abscessus isolate appeared to be resistant to all antibiotics (TDR), while two isolates of M. chelonae showed resistance to ethambutol and rifampicin, while M. smegmatis showed weak resistance to pyrazinamide and resistance to rifampicin. Also, all isolates of M. chelonae were sensitive to pyrazinamide, isoniazid and streptomycin.

Conclusion: It appears that the rapidly growing mycobacteria represents a high frequency, among nontuberculosis detected patients, which requires phenotypical and genotypical confirmation on a follow-up, along with the examination of patterns of sensitivity. Implementing Duplex-PCR proved to be decisive for differentiating NTM from MTB.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Amin A. Al-Sulami, Asaad Al-Taee and Zainab A. Hasan

Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version