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Commentary Open Access
The higher order structure of human chromosomes remains to be elucidated with the 30 nm mystery still remaining. For internal structure determination, transmission electron microscopy (TEM) cannot be used as the chromosomes are too thick (approx. 1.4 microns) and scanning electron microscopy (SEM) is a surface imaging technique. For this purpose, the three-dimensional (3D) serial block face scanning electron microscopy (SBFSEM) was used on imaging mitotic human chromosome for the first time .
Chromosome, Serial block-face scanning electron microscopy, Three-dimensional imaging, Single Cell Analysis,Single cells