alexa Abstract | Hypericum triquetrifolium Extracts Modulate IL-6, IL-10 and TNF-α Protein and mRNA Expression in LPS-Activated Human Peripheral Blood Mononuclear Cells and THP-1-Derived Macrophages
ISSN: 2167-0412

Medicinal & Aromatic Plants
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

The aim of the present in vitro study was carried out to evaluate the anti-inflammatory activity of Hypericum triquetrifolium extracts (HT-extract) on lipopolyssacharide -stimulated human monocytic (THP-1) cells and human peripheral blood mononuclear cells (PBMNCs). The expression and production of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6), as well as the anti-inflammatory cytokine interleukin 10 (IL-10) were evaluated by assessing the levels of proteins and mRNA’s of TNF-α, IL-6 and IL-10 in both cell types. Cells were exposed to 5 μg lipopolyssacharide (LPS) /ml in the absence and presence of increasing concentrations of 50% ethanol extracts from the aerial parts of Hypericum triquetrifolium. The anti-inflammatory efficacy experiments were performed with HT-extract concentrations up to 250 μg/ml that had no cytotoxic effects as assessed with MTT and LDH assays. HT-extract remarkably inhibited the expression and secretion of TNF-α and IL-6 at a concentration of 250 μg/ml. HT-extract remarkably elevated IL-10 secretion and mRNA levels at 125 μg/ml. Furthermore, HT-extract exhibited relatively high antioxidant activity (IC50 of 5 μg/ml) as measured with DPPH assay. These findings indicate that HT-extract probably exerts anti-inflammatory effects at both protein and gene expression levels of the anti-inflammatory as well of the pro-inflammatory cytokines in PBMNCs.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Saad B, Embaslat WH, Farich BA, Mahajna S, Azab M, Daragmeh J, Khasib S and Zaid H

Keywords

Anti-inflammatory, Hypericum triquetrifolium, PBMNCs, Medicinal plants, Chinese Medicine, Clinical Naturopathic Medicine, Clinical Naturopathy

Share This Page

Additional Info

Loading
Loading Please wait..
 
 
 
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords