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Research Article Open Access
Arabidopsis plasma membrane nanodomains have been visualized in vivo using Remorin proteins of the taxonomic group 1b. The physiological role of such domains is to facilitate protein-protein interactions near the plasma membrane and to act as signal transducing platforms, routing information from the extra- to the intracellular side of the cell. Beneath sterols and sphingolipids as lipid components, group 1b Remorin proteins showed a strong tendency to accumulate in nanodomains. In contrast to sterol- and sphingolipid enriched membrane domaincounterparts in animals, individual Arabidopsis nanodomains did not exhibit any kind of lateral movement in the plane of the membrane. Since group 1b Remorins are reported to form microfilaments in vitro, assemblies of Remorin microfilaments with stable structures could have an impact on domains´ lateral stability in the plane of the membrane. To test a possible influence of cell wall components on the mobility of individual Remorin proteins resident in plasma membrane nanodomains FRAP-experiments were performed in Arabidopsis mesophyll tissue and protoplasts, using fluorescently labeled group 1b Remorins. It was revealed that the cell wall has a stabilizing effect on the mobility of individual Remorins resident in nanodomains.
Plasma membrane, Nanodomain, Remorin, FRAP, Protoplast, Plant Biotechnology,Plant Biochemistry,Green Chemistry,Enzyme Activity,Transcriptomics,Nucleic Acid,Germination,Phytochemical