Journal of Carcinogenesis & Mutagenesis

Abstract

Inhibitory Effects and Anti-Invasive Activities of Trehalose Liposomes on the Proliferation of Lung Carcinoma Cells

Keiji Kuwabara, Hideaki Ichihara and Yoko Matsumoto

Inhibitory effects and anti-invasive activities of trehalose liposomes (DMTreC14) composed of L-α-dimyristoylphosphatidylcholine and α-D-glycopyranosyl-α-D-glucopiranoside monomyristate (TreC14) on the proliferation of human non-small cell lung carcinoma (A549) cells were examined in vitro. DMTreC14 with a hydrodynamic diameter less than 100 nm, were preserved for 4 weeks. The inhibitory effects of DMTreC14 on the proliferation of A549 cells accompanied with apoptosis were obtained. Enhancement of the accumulation of DMTreC14 into A549 membranes was observed. An increase in cellular membrane fluidity of A549 cell treated with DMTreC14 was observed on the basis of fluorescence depolarization method. DMTreC14 caused apoptosis for A549 cells through the activation of caspases and mitochondrial pathway. The anti-migration effects of DMTreC14 for A549 cells were observed through the inhibition of filopodia formation. The anti-invasion effects with the reduction of MMP-14 of DMTreC14 for A549 cells were obtained.