alexa Abstract | Pattern of Keratin Expression and its Impact on Nuclear-Cytoplasmic Ratio in Plump Keratinized Squamous Cells during Oral Carcinogenesis
ISSN: 2168-9784

Journal of Medical Diagnostic Methods
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

Background: Cellular alteration is a unique feature during multi-step oral carcinogenesis. Non-keratinized normal oral squamous cells get keratinized in order to protect and preserve their normalcy in both structure and function. But, the pattern of keratinization seriously affects the nuclear-cytoplasmic ratio of the concerned cells. In the present study, the pattern of keratin expression and its impact on nuclear-cytoplasmic ratio in plump keratinized squamous cells during carcinogenesis was vividly analysed.

Materials and Methods: Samples in the form of scraped and exfoliated cytosmear were collected from the affected sites of the clinically diagnosed 136 oral cancer patients and were immediately fixed in acetoalcohol (1:3). The wet fixed smears were stained by routine Papanicolaou’s staining protocol and Giemsa’s solution. Stained tissues were studied under the microscope.

Results: Cytological pleomorphism is a unique feature observed during oral carcinogenesis and pattern of keratin expression in these pleomorphic cell reflect the cellular pathogenecity. Huge keratin expression in the cytoplasm of the plump keratinized squamous cells is presumed be to an important cause of alteration in the architectural regularity of the cell membrane which lead to cytological pleomorphism as well as a significantly (p<0.01) increase in nuclear- cytoplasmic (N/C) ratios from precancerous (1:27.9 in male and 1:28.9 in female) to cancerous (1:17.8 in male and 19.6 in female) cases during oral carcinogenesis.

Conclusion: Pattern of keratin expression in well differentiated PKSCs is presumed to be a factor of cytological pleomorphism and increase in N/C ratio from precancerous to cancerous cases. Also, the appearance of PKSCs may be considered as a sign and index of cellular alteration in oral epithelia and a progenitor of cytological pleomorphism during oral carcinogenesis. And thus, the present finding has a practical utility in the field of diagnostic cytopathology in general and early detection of oral cancer during carcinogenesis in particular.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Mohanta A, Mohanty PK

Keywords

Adrenocortical Carcinoma, Clinical Diagnostic Test, Differential Diagnosis, Laboratory Diagnosis

Share This Page

Additional Info

Loading
Loading Please wait..
 
 
 
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords