alexa Quantitation of chlorogenic acid from aqueous and methanolic extracts of wood apple (Aegle marmelos C. Linn) by GC-MS Ion – trap | Abstract
ISSN ONLINE(2319-8753)PRINT(2347-6710)

International Journal of Innovative Research in Science, Engineering and Technology
Open Access

Like us on: https://twitter.com/ijirset_r
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

GCMS is a robust and very widely used technique that combines high sensitivity and specificity for suitable chemical constituents. The identification and quantitation of chemical constituents has been done by GC/MS Ion-trap with respect to the internal and external standard. The internal standards are 60000 patterns of reference compounds and its reference spectrum of NIST library. The present study has been conducted for the quantification of chlorogenic acid (CGA) from the aqueous and methanolic extracts of wood apple (A.marmelos) with external standard by GC-MS Ion trap. Chlorogenic acid is a targeted compound, water soluble phenolic compound it plays vital role in physiological functions in plants and pharmacological activities in humans. The total mass of chlorogenic acid is 354. It would be identified and quantified from different extract of wood apple in comparison with external standard. Retention time of standard chlorogenic acid, aqueous extract, methanolic extract and combined extracts indicates 43.237, 43.236, 43.234 and 43.241 minutes respectively. Mass spectral data of standard fragmented ions are 311.3, 312.3, 325.3, 354.2, 355.0 and these ions were found in all the samples of wood apple. Therefore, quantitation of target compound with respect to the external standard, its similarity of retention time and mass spectral data between the standard and samples would be used for the identification and quantification of target compound. The targeted compound has been quantified as 41.9, 9.5 and 29.7 percent of aqueous extract of WA, methanolic extract of WA and combined extract of WA respectively. GCMS is a selective and sensitive analytical method to identify and quantify the target compound in chemical matrix with reference to internal and external standard of chemical constituents. Target component possess wide application of medicinal properties, especially in psychiatric disorders and this analysis might be used to know the exact concentration of target chemical components in the aqueous and methanolic fruit extract of A.marmelos (wood apple).

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): C.Rajeshkannan and G.Lakshmanan

Keywords

Chlorogenic acid, Wood apple (WA), A.marmelos, target ions, TIC and Mass spectral data., GC-MS

Share This Page

Additional Info

Loading
Loading Please wait..
 
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsonline.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsonline.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsonline.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsonline.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsonline.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsonline.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsonline.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version