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Research Article Open Access
A spectrophotometric method has been developed for the determination of tebuconazole fungicide formulation. The method is based on the complexation reaction of ferric chloride with tebuconazole fungicide in acidic media and the purple colored complex was studied at 530 nm. The reaction conditions and other analytical parameters were optimized. A linear calibration curve between absorbance and concentration over the range from 0.2 to 20 μg mL−1 was obtained with molar absorptivity of 2.1 × 104 L mol−1 cm−1. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.05 and 0.2 μg mL−1 respectively. The proposed method has been successfully applied for the analysis of commercial formulation of tebuconazole fungicide. The recoveries of the method were found to be in the range of 92.16 ± 0.06% to 96.66 ± 0.18%.
Ferric chloride, Sodium hydroxide, Tebuconazole fungicide, UV/Visible spectrophotometer, Chemical science and Spectrophotometry