alexa Abstract | STAPHYLOCOCCUS AUREUS in DAIRY ANIMALS and FARM WORKERS in a CLOSED HERD in KARNAL, NORTH INDIA: ASSESSMENT of PREVALENCE RATE and COA VARIATIONS
ISSN ONLINE(2319-8753)PRINT(2347-6710)

International Journal of Innovative Research in Science, Engineering and Technology
Open Access

Like us on: https://twitter.com/ijirset_r
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

In order to isolate and characterize S. aureus from milk of dairy animals and nasal swabs of farm workers, the present study was conducted on a total of 200 milk samples from lactating animals (including 50 Murrah buffaloes, 90 indigenous Sahiwal and 60 crossbred Karan Fries cattle) and 50 nasal swabs of farm workers in an organized dairy farm in Karnal, North India. The collected samples were cultured on mannitol salt agar and presumptive S. aureus colonies were confirmed phenotypically (coagulase, catalase and indole test) and genotypically (PCR amplification of nuc gene). Genotypic variation among S. aureus isolates from different sources was studied by amplifying the 3’ hypervariable region of the coagulase gene. 73.6% of all the samples, 74.5% of the milk and 70% of the nasal swabs were tested positive. Highest prevalence rate was observed in milk from crossbred cattle (88.33%) followed by indigenous cattle (80%) and buffalo (48%). 89.74% of previously treated animals and 53.01% of the healthy animals were tested positive. Amplification of the coagulase gene from the milk isolates produced a single PCR product of 600- bp whereas the nasal swab isolates produced five different PCR products of sizes 600 (10 isolates), 680 (14 isolates), 790 (8 isolates), 950 (1 isolate) and 1000-bp (1 isolate). Absence of variation among the milk isolates shows the importance of maintaining a close herd to prevent the entry of new S. aureus strains in the herd. However, the common existence of 600-bp genotype indicates transmission of the isolates between the species.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Purba Sarkar , Debasish Mohanta, Sachinandan De, Chanchal Debnath

Keywords

Staphylococcus aureus, Prevalence, Mastitis, Coagulase, Genotype, Staphylococcus Aureus

Share This Page

Additional Info

Loading
Loading Please wait..
 
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords