Objective: To explore the effect of laparoscopy on cancer cells and the expressions of adhesion molecules (ICAMÃ¢ÂÂ1, CD44v6 & integrin β1) in peritoneal tissue and abdominal rinse in patients with colorectal carcinoma
Methods: A total of 65 CC cases undergoing radical resection at our hospital were analyzed. They were divided into two groups of LAP (laparoscopy, n=35) and OP (open surgery, n=30). Peritoneal tissues were collected at incision and beyond .Also abdominal rinse was collected before tumor resection and abdomen closure. The expressions of adhesion molecules (ICAMÃ¢ÂÂ1 ,CD44v6 & integrin beta 1) in peritoneal and abdominal cavities of two groups were detected by immunohistochemistry and doubleÃ¢ÂÂantibody sandwich ABCÃ¢ÂÂELISA. And cancer cells
in abdominal rinse were detected by peritoneal lavage cytology
(PLC) for comparing two surgical methods.
Results: In LAP group, PLC was positive in 3 cases (8.6%) before tumor resection and 8 cases (22.9%) before abdominal closure. In OP group, PLC was positive in 4 cases (13.3%) before tumor resection and 8 cases (26.7% ) before abdominal closure. No interÃ¢ÂÂgroup difference existed in PLC (P>0.05). The expressions of CD44v6, ICAMÃ¢ÂÂ1 and integrin beta 1 in abdominal cavity (before tumor resection & abdomen closure) were compared for LAP and OP groups. And there was no statistically significant difference ( P>0 05). The expressions of CD44v6 ,ICAMÃ¢ÂÂ1 and integrin beta 1 in peritoneal tissues (at incision and beyond) were compared between LAP and OP groups .And there was no statistically significant difference (P>0.05) .
Conclusion: Compared with CC patients undergoing traditional open surgery, the risk of exfoliated cancer cells in abdominal cavity shows no increase. And there is no impact `upon the expressions of adhesion molecules.
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Author(s): Xin Wang, Anren Sun, Linkang Xiao and Rong Yang