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Analytical Chromatography Journals has become a cornerstone of separation science, that branch of chemistry dedicated to separating compounds from mixtures. Analytical chromatography uses little sample sizes; the target is to separate compounds so as to spot them The mobile phase of chromatography refers to the fluid that carries the mixture of substances in the sample through the adsorptive material. The stationary or adsorbent phase refers to the solid material that takes up the particles of the substance passing through it. Kaolin, alumina, silica, and activated charcoal have been used as adsorbing substances or stationary phases.