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Ion-exchange chromatography retains analyte molecules on the column based on coulombic (ionic) interactions. The stationary phase surface displays ionic functional groups (R-X) that interact with analyte ions of opposite charge. This type of chromatography is further subdivided into cation exchange chromatography and anion-exchange chromatography. The ionic compound consisting of the cationic species M+ and the anionic species B- can be retained by the stationary phase. The most popular method for the purification of proteins and other charged molecules is ion exchange chromatography. In cation exchange chromatography positively charged molecules are attracted to a negatively charged solid support. Conversely, in anion exchange chromatography, negatively charged molecules are attracted to a positively charged solid support.
Related Journals of Ion-exchange chromatography
Journal of Chromatography & Separation Techniques, Journal of Analytical and Bioanalytical Techniques, Pharmaceutical Analytical Acta, Journal of Separation Techniques.