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Pure Microbiology is a branch deals with application of microorganisms in the field of science for the production of human beneficial products such as medicines, antibiotics, vaccines, enzymes, biotechnological products. Micro-organisms possess membrane-bound cell organelles and include fungi and protists, whereas prokaryotic organisms all of which are microorganisms are conventionally classified as lacking membrane-bound organelles and include eubacteria and archaebacteria. Microbiologists traditionally relied on culture, staining, and microscopy.