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Journal of Next Generation Sequencing & Applications

ISSN 2469-9853

Journal of Next Generation Sequencing & Applications

Sanger Sequencing

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Sanger Sequencing

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Sanger sequencing is defined as a method of DNA sequencing which is based on incorporation of chain- terminating dideoxynucleotides by DNA polymerase during in vitro  DNA replication. Sanger sequencing by capillary electrophoresis is the gold-standard DNA sequencing technique that is used in a number of experimental workflows in life sciences laboratories. In Sanger sequencing, DNA polymerases copy single-stranded DNA templates by adding nucleotides to a growing chain. Chain elongation occurs at the 3' end of a primer, an oligonucleotide that anneals to the template. The deoxynucleotides added to the extension product are selected by base-pair matching to the template.

Related journals for Sanger Sequencing

Journal of Next Generation Sequencing & Applications, Advancements in Genetic Engineering, Journal of Computer Science & Systems Biology, Journal of Proteomics & Bioinformatics, Transcriptomics: Open Access, Frederick Sanger sequencing Journal, Sanger Sequencing Biosystems, Methods of Sanger Sequencing, Nucleic acids Research Oxford Journals.

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