Sri Venkateswara Veterinary University, India
B Swathi currently working in Sri Venkineswara Veterinary University, India. Her research interet is Efficacy of tulsi and turmeric as antioxidants
A study was undertaken up to investigate the efficacy of herbals tulsi (Ocimum sanctum) and turmeric (Curcuma longa) in ameliorating the oxidative damage induced by heat stress with 216 day old vencobb broiler chicks in 2 batches in hot summer months. In each batch, 108 chicks were divided into 9 groups, consisting of 12 chicks in each group. Group (G–I) was given basal diet (BD) without any antioxidant. While G-II was given BD with vitamin E (200 mg/kg) supplement and for G-III selenium was supplemented @ 0.15 mg/kg along with vitamin E (200 mg/kg). Group IV, V, VI and VII were given tulsi (0.25%), tulsi (0.5%), turmeric (0.2%) and turmeric (0.4%), respectively as supplements to BD. Combination of both herbals were supplemented with BD in G VIII [(tulsi (0.25%) +turmeric (0.2%)] and G-IX [(tulsi(0.5%) +turmeric (0.4%)]. Additionally, a control group of 12 chicks was raised separately in stress free environment. Plasma samples were obtained at 4th and 6th wk of age and assessed for enzymatic (glutathione peroxidase (GSH_Px), superoxide dismutase (SOD) and catalase and non enzymatic (reduced glutathione (GSH)) antioxidants status. The data generated from the 2 batches was averaged and analysed. Heat stress significantly (P≤0.01) reduced the antioxidant status in heat stressed birds fed on BD without any antioxidant supplementation compared to control birds. Among all the antioxidant supplemented groups, Se along with vitamin E had shown higher (P≤0.01) antioxidant status, followed by vitamin E inclusion. Herbals tulsi and turmeric improved the antioxidant status. Higher inclusion levels of tulsi (0.5%) and turmeric (0.4%) were effective (P≤0.01) in improving the antioxidant status than when supplemented with lower doses (0.25% and 0.2% respectively). Combination of herbals (G-VIII and IX) had not shown any additional benefit than their independent inclusions. The present study indicated that herbals tulsi and turmeric supplemented at 0.5 and 0.4% levels, respectively in broiler diets could be used as safe alternatives to synthetic antioxidants to combat heat stress.
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