Bakrudeen Ali Ahmed
University of Malaya
Dr. Bakrudeen Ali Ahmed, working as Senior Lecturer in the Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia. His research areas include the application of plant propagation, bio-active compound production via Plant tissue culture methods. Development of pharmaceutical products and pharmacological studies. He is Member of IABMS (Indian Biomedical Scientist Association) and MSPP (Malaysian Society of Plant Physiology). Indian Biomedical Association has given him the Agathiyar Award 2007 for his work. He has received bachelor’s degree (Chemistry), Master degree (Biochemistry) and Ph.D. (Plant Science-Biochemistry) from Bharathidasan University, Tiruchirappalli, Tamil Nadu, India. He has 3 years Postdoctoral Fellowship experiences in Pukyong National University, Busan, South Korea and University of Malaya, Kuala Lumpur, Malaysia. He has published 47 publications, including the 17 ISI publication, 14 Book chapters etc.
Obesity is one of the fastest-growing major diseases in many area of the world including Europe, the United States and Japan. Gymnema sylvestre (Asclepiadaceae) is well-recognized in traditional medicine as a remedy for diabetes mellitus and used in folk, ayruvedic, and homeopathic systems of medicine. Apart from its other traditional uses, in the leave extracts of G. sylvestre has been used to control diabetes and dyslipdaemia.Optimum callus was developed under stress conditions of blue light with 2,4-D (1.5 mg/l) and KN (0.5 mg/l), which induced maximum biomass of green compact callus at 45 days, as determined by growth curve analysis. Evaluation and isolation of gymnemic acid from the calluses derived from different plant parts, namely, leaf, stem and petioles have been done in the present case for the first time. Factors such as light, temperature, sucrose, and photoperiod were studied to observe their effect on gymnemic acid production. Leaf and optimum callus extracts contains gymnemic acid detection and quantification were carried out using thin-layer chromatography (TLC), highperformance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), and gravimetric techniques. GA detection peak area and their absorption spectra were evaluated through HPTLC and HPLC with the standard GA.The rats were bled at the beginning of the experiment and at 10 days intervals. Blood glucose, cholesterol, triglyceride, very-low density lipoprotein (VLDL), low-density lipoprotein (LDL) and high-density lipoproteins (HDL) were estimated. Results showed that G. sylvestre leaf and blue-induced in vitro callus extracts reduced triglyceride, VLDL, LDL levels and increased HDL level. The gymnemic acid of leaf and callus methanolic extracts of G. sylvestre for its glucose and lipid regulating activities in high-fat diet-fed rat model of metabolic syndrome, when compared with the standard high-fat diet-fed rats. It could have potential as a pharmaceutical drug for obesity.