Carlos Alberto Alves da Silva
Catholic University of Pernambuco
Carlos Alberto Alves da Silva is PhD in Biotechnology by the Technical University of Lisbon - Instituto Superior Técnico (1998) and Post Doctorate at the Institute of Chemistry Sarria - Ramon Llull University, Barcelona (2007-2008). He is currently Associate Professor III and teaches courses in the Environmental and Chemical Engineering, Chemistry Degree and Effective Researcher at the Nucleus for Research in Environmental Science and Biotechnology (NPCIAMB, Catholic University of Pernambuco).The main research activities in Biotechnology, Industrial and Environmental Microbiology are: Bioprocess of Separation, Isolation/Identification of Microorganisms, Production and purification of microbial enzymes.
Enzymes are more biotechlogical products currently used in several industrial sectors, but also have high costs of production and purification. Studies to reduce the production processes through the formulation of alternative media using low cost components and the discovery of new microorganisms producers. Lipases (triacylglycerol acylhydrolases EC 220.127.116.11) are important industrial enzymes due to their versatile applications. The agro-industrial wastes are chemical compounds derived from various industries, which often have high nutritional potential, but are often discarded in the environment without any treatment. The Caatinga is a large geographic area in Brazil comprising a variety of different types of vegetation and extensive microbial biodiversity little studied. The use of factorial design in fermentation processes has emerged as a viable alternative because it reduces the number of experiments. Studies for lipase production were performed using a 23 factorial design to select the best condition for enzyme production. The assays occurred in 144 hours 28oC, 150 rpm. The assay 7 had the highest lipolytic activity obtained which was 101.2 U / mL. The rice bran, wheat bran and frying oil, keeping the same initial conditions of the fermentation process used in the factorial design. After selecting the best condition, alternative media containing agro-industrial wastes were produced. The results indicated that the medium containing frying oil had the highest lipase activity, whose value was 276 U/mL. The utilization of waste agro-industrial from the food industry has as viable alternative for development of the media of production of metabolites with high biotechnological potential.