Ebtesam M. Al-Ali
Kuwait institute for scientific research, Kuwait
Ebtesam M. Al-Ali has completed her BSC Studies at 1993 from Kuwait University and joined the work as scientific researcher at Kuwait institute for scientific research with the molecular genetics group, she led 5 projects, 3 completed and 2 ongoing, she has published more than 5 papers in reputed journals and international conferences.
Farmers complained to the Kuwait Institute for Scientific Research (KISR) about diseases affecting their vegetable crops, leading to subsequent heavy economical losses. Initial field visits indicated that the diseases might be caused by viruses. Four plant families, including Cucurbitaceae (cucumber, squash, melon, zucchini), Solanaceae (tomato, potato, pepper, eggplant), Liliaceae (onion) and Leguminosaea (bean) were surveyed throughout the growing seasons for viruses by ELISA from December through May. The survey was conducted on six farms located in the two main agricultural districts areas, Wafra (South) and Abdally (North). Each farm was visited four times during the production season (planting, pre-flowering, post-flowering and fruiting). This preliminary survey constitutes the first report in Kuwait of fifteen out of eighteen viruses detected, including Cucumber mosaic virus, Garlic common latent virus, Iris yellow spot virus, Onion yellow dwarf virus, Melon necrotic spot virus, Papaya ringspot virus, Pepino mosaic virus, Pepper mild mottle virus, Potato moptop virus, Potato virus M, Potato virus X, Potato virus Y, Squash mosaic virus, Tomato mosaic virus, Tomato spotted wilt virus, Tomato yellow leaf curl virus, Watermelon mosaic virus, and Zucchini yellow mosaic virus. Virus incidence was close to 100% on some crops, including cucurbit and onions and double or triple infections were common. Several important viruses were not found, including Potato virus A, Potato virus S, Potato leafroll virus, and Potato virus V. TYLCV was also detected by PCR, and partial sequence of the amplicon was done.
Keywords: ELISA (Enzyme Linked Immunosorbant Assay), Cucurbitaceae, Solanaceae, Liliaceae, Leguminosaea.
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