Euis Holisotan Hakim

Euis Holisotan Hakim

Bandung Institute of Technology, Indonesia

Title: Exploration of Morus plant as a source of bioactive chemicals


"Professor Dr. Euis Holisotan Hakim (10 May 1953) received a Bachelor’s degree in Chemistry from Institut Teknologi Bandung (1980) and she joint the research group of Professor Sjamsul Arifin Achmad at ITB, She received Masters degree ( 1989) and a Ph.D. (1994) from the same University. She got a research Fellow at the University of Tokyo (1988), DSIR, New Zealand (1991), University of Western Australia (1992), and post doctoral research with Prof. Shigeo Iwasaki, the University of Tokyo (1996) and with Prof. Takeya at Tokyo University of Pharmacy & Life Science (2000). She is promoted to associate professor in 2000 and as full professor in 2004. Research interests: bioactive natural product compounds for anti tumor and anti malaria; Development of plant tissue culture for secondary metabolite production and combinatorial biosynthesis. "


In our search for bioactive compounds from Indonesian tropical plants, a phytochemical investigation of several species of moraceous plants has been undertaken in our laboratory. Morus is one of the most important genera belongs to Moraceae family, this genus consist of 15 species where the leaf of this plant are very popular for feeding of silk worm. Most of these species are used as traditional medicine in many places and well known as “sohakuhi” in Japan and “sangbaipi” in China. Phenolic compounds isolated from Morus mainly stilbenoid and arylbenzofuran derivatives in addition to flavonoid and Diels-Alder type adducts, some of which are exhibited interesting biological activity including anti-tumor. Development of root culture of M. macroura yielded mostly Diels-Alder type adduct compounds, while shoots culture of this species produce a prenylated chalcones which identified as dienophile founded in Diels-Alder type adduct of Morus. The root culture of M. cathayana afforded O-methylated Diels-Alder adduct compounds which were secreted to the media. Further elicitation of root culture of M. macroura by abiotic and biotic elicitor increased the production of secondary metabolite significantly. Further investigation of enzyme which responsible on Diels-alder adducts production of Morus plant showed a promising data for combinatorial biosynthesis study. And recently we try to developed endophyte microbe of Morus, some strains of fungi content potential secondary metabolites.