Fan Yang and Qiang Lin Duan
TongJi University School of Medicine, China
Fan Yang has graduated from DongNan University School of Medicine. He is the Deputy Director of Clinic Laboratory in TongJi Hospital which belongs to TongJi University School of Medicine. He has published more than 20 papers in reputed journals and has been studying for over 15 years in clinical immunology.
QiangLin Duan has graduated from TongJi University School of Medicine. He is a doctor of department of cardiology in TongJi Hospital which belongs to TongJi University School of Medicine. He has published more than 20 papers in reputed journals and has been studying for more than 5 years in the project of new strategies in prevention and treatment of venous thromboembolism.
To investigate localization and distribution of integrin subunit beta1, beta2 and beta3 and morphological changes of ligand-recepter binding in thrombi of acute pulmonary embolism (PE) patients and explore activation of circulated immune cells, inflammatory immune adherence and coagulation response in acute venous thrombosis. In this study, we found: 1) Acute venous thrombi were red thrombi composed of skeletons and filamentous mesh containing large amounts of red blood cells and white blood cells; 2) Integrin subunit beta1, beta2 and beta3 were expressed on lymphocytes, neutrophils and platelets; 3) No expression of integrin beta1 ligands: Laminin, Fibronectin, Collagen I or Collagen-II on lymphocytes; integrin beta2 ligands including ICAM, factor X and iC3b are distributed on neutrophils, and ligand fibrinogen bound to neutrophils; integrin beta3 was expressed on platelets which form the skeleton of thrombi and bound to fibrinogen to construct mesh structure; 4) Factor Xa was expressed on the filamentous mesh; 5) Filamentous mesh was fully filled with red blood cell dominant blood cells. So we can conclude that acute venous thrombosis is an activation process of circulated lymphocytes, neutrophils and platelets mainly, and a whole process including integrin subunit beta2 and beta3 binding with their ligands. Activation of immune cells, inflammatory immune adherence and coagulation response are involved in the acute venous thrombosis.