University of Science and Technology of China, China
Gaolin Liang, born 1972, is full professor at University of Science and Technology of China starting from March 2010. He received his B.S. from Nanjing University in 1993, M.S. from Zhengzhou University in 2002, and Ph.D. from Fudan University in 2005. From 2005 to 2008, he was a postdoctoral fellow at The Hong Kong University of Science and Technology under the supervision of Professor Bing Xu. From 2008 to 2010, he was a postdoctoral fellow at Stanford University under the supervision of professor Jianghong Rao. Prof. Liang’s research interests mainly focus on nanochemistry, molecular and cellular imaging, and biomedical analysis.
Herein, we report the development of a new fluorescence switch for selectively sensing Cu2+ and L-Histidine (L-His) in vitro and in living cells for the first time. In the absence of metal ions, Ac-SAACQ-Gly-Gly-Gly-Lys (FITC) (1) exhibits comparable fluorescence to that of free FITC. In the presence of metal ions, 1 selectively coordinates with Cu2+, causing its fluorescence emission quenched via photoinduced electron transfer. Interestingly, as-formed 1-Cu2+ complex selectively responds to L-His among the 20 natural amino acids by turning its fluorescence on. This property of fluorescence switch of 1 was successfully applied to qualitatively and quantitatively sensing Cu2+ and L-His in vitro. Using this dualfunctional probe, we also sequentially imaged Cu2+ and L-His in living HepG2 cells. Our new probe 1 could be applied for not only environment monitoring or biomolecule detections, but also disease diagnoses in the near future.
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