National Research Centre on Seed Spices, India
Geetika Jethra is a Senioe Research Fellow at National Research Centre on Seed Spices, India
In-silico structural identification is a new breakthrough through which we can unearth the structural and functional parameters of the plant protein. Fenugreek (Trigonella foenum-graecum L., 2n=2x=16) is an important leguminous spices and well known aromatic and medicinal herb. Fenugreek is used as both seed and leaf. Fenugreek seed contains carbohydrate (48%), protein (25.5%), mucilaginous matter (20%), fat (7.9%), and saponin (4.8%). In the present study, FENUGREEK (Trigonella foenum-graecum) protein models were designed and generated using in-silico tools. The identified protein of fenugreek showed homology with the protein domain of humans and E-coli illustrating that the database available on Apiaceae family is sparsely populated with sequences. The estimated molecular weight of identified fenugreek AM-1 protein was 11372.8 and was predicted as a basic protein with computed pI 10.38. Aliphatic index determined for the protein sequence was 68.82 portraying it to be thermally stable and globular. We have first time identified this channel in fenugreek transmembrane protein, through which a ligand (an ion or a small molecule) might pass. The results revealed that random coil (69.61%) dominated among secondary structure elements and alpha helices (4.90%) and extended strand (25.49%) were also present. Further validation can be performed using wet lab experiments. But, the present finding may be very much valuable in addition to the proteomic information available on fenugreek and it can be used in ensuing breeding programs for development of improved varieties.
Keywords: Fenugreek, Apiaceae, Aliphatic index, random coil and alpha helices.
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