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Biography

Hyo-Ihl Chang has completed his PhD in 1987 from North Carolina State University. He has been professor of College of Life Sciences and Biotechnology, Korea University since 1988. He was a dean of College of Life Sciences and Biotechnology, Korea University. He has published more than 80 papers in reputed journals. Now he is a president of the Korean Society for Microbiology and Biotechnology.

Abstract

Bacteriophages can be operated as highly immunogenic antigens, which could interact with dendritic cells as antigen presenting cells. The bacteriophage ESP2949-1 is a lytic phage of Cronobacter sakazakii which has been isolated from sewage samples. Unlike other phages that were researched as therapeutic agents for many diseases, the ESP2949-1 phage has never been clearly examined for therapeutic purpose. To evaluate its therapeutic effect, the production of the proinflammatory cytokines TNF-α, IL-6, IL-1α, and IL-1β, the expression of the dendritic cell maturation markers CD86 and CD40, and the underlying of NF-κB signaling pathways in murine bone marrow-derived dendritic cells (BM-DCs) in response to ESP2949-1 phage infection were studied. The bacteriophage ESP2949-1 affected the expression of the cell surface molecules and proinflammatory cytokines that are related with the DC maturation processes. Treatment with ESP2949-1 phage also induced the NF-κB-IL12p40 signaling pathways. Our chromatin immunoprecipitation assay(ChIP) showed that p65 could bind the IL12-p40 promoter via translocation to the nucleus which indicates the activation of NF-κB signaling. Furthermore, the ESP2949-1 phage induced the promoter activity of IL-12p40. Our ChIP assay also revealed that p65 was enriched at the IL12-p40 promoter as a direct target of chromatin. The present study demonstrates that the ESP2949-1 phage potently induces DC maturation via immune-enhancement processes.