VaxInnate Corporation, USA
Langzhou Song isDirector of Molecular Biology and Protein,Science at VaxInnate Corporation.Greater New York City AreaBiotechnology
Previously, we demonstrated that for H1N1 and H5N influenza strains, the globular head of the hemagglutinin (HA) antigen fused to flagellin of Salmonella typhimurium fljB (STF2) is highly immunogenic in preclinical models and man (Song, et al., Plos One 2008; 3(5):e2257; Song, et al., Vaccine 2009, 27(42):5875; Taylor, et al., Vaccine 2012, 30(39):5761). Further we showed that the vaccine format, or point of attachment of the vaccine antigen to flagellin, can dramatically affect the immunogenicity and safety profile of the vaccine. However, Influenza B vaccines based on these formats are poor triggers of TLR5 and consequently are poorly immunogenic. Through rational design, here we show that we have identified a fusion position within domain 3 of flagellin that improves TLR5 signaling and consequently, immunogenicity of multiple influenza B vaccines. Our results demonstrate that, similar to influenza A strains, the protective subunit of the influenza B HA can be fused to flagellin and produced in a standard prokaryotic expression system thereby allowing for cost and time efficient production of multivalent seasonal influenza vaccines.